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Project | 11
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Whole Exome Sequencing of RPE1 cells with p53, BRCA1 and TP53 knock-outs

Summary

Synthetic lethality (double-hit) phenomena are very useful in identifying druggable targets with cell-type precision. In this project, RPE1 cells with p53, BRCA1 and TP53 knock-outs were created using the CRISPR-Cas9 method. To investigate the off-target effects of the CRISPR-Cas9, WES was done on the Illumina Selexa platform. The data analysis was done using FastQC, BWA, Picard, SAMtools, BCFtools, snpEff and SnpSift tools

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