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Gopal Krishna Ramadas Dhondalay PhD
Data Scientist / Bioinformatician / Computational Biologist / Human Geneticist
Project | 07
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Her-2/neu overexpression due to polysomy 17 in breast cancer: molecular testing to guide therapeutic options
Summary
Fluorescent in situ hybridisation (FISH) equivocal results for Her-2/neu still pose a diagnostic dilemma in oncology practice. In this study, we evaluate whether Her-2/neu mRNA expression is an alternative to FISH for detecting Her-2/neu positivity. Archival paraffin blocks of 54 breast cancer patients were analysed for Her-2/neu status using immunohistochemistry (IHC), FISH, and Her-2/neu gene expression using mRNA. There was a 100% positive agreement and 64.7% negative agreement of Her-2/neu mRNA expression with respect to the reference standard (FISH), with the kappa value for agreement being 0.36. mRNA levels correlated positively and strongly with FISH ratio and IHC positivity. For Her-2/neu mRNA expression, Her-2/neu copy number was a significant predictor, indicating that mRNA expression is independent of polysomy status.
A publication in the Journal of Onkologie was published based on this project.
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