Gopal Krishna R Dhondalay PhD

Summary

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Soil acts as a vital source of nutrients for growing plants. It also composed of antibiotics which give protection during the development stages. In an attempt to isolate these antibiotics from soil, we collected soil samples from various places in Bangalore city, India. To develop cultures of microorganisms growing present in soil, we serially diluted soil solution and cultures. The colonies developed ion agar plates were counted and the growth rate were measured. The antibiotics were from broth culture and simultaneously the colonies were characterized for their identification. The Actinomycetes and Streptomycetes colonies were characterized and antibiotics were isolated and purified by activated charcoal method. The reactivity of the antibiotics were tested against commercially available grade of antibiotics. As a result, we were able to isolate and characterize microbial colonies from soil. The reactivity of purified and isolated antibiotics were equivalent to commercially available antibiotics. This was an in-house developed project for the partial fulfillment of bachelor's degree.