Gopal Krishna Ramadas Dhondalay PhD

Summary

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Soil acts as a vital source of nutrients for growing plants. It is also composed of antibiotics, which give protection during the development stages. In an attempt to isolate these antibiotics from soil, we collected soil samples from various places in Bangalore city, India. To develop cultures of microorganisms growing in soil, we serially diluted the soil solution and cultures. The colonies developed on agar plates were counted, and the growth rate were measured. The antibiotics were from broth culture, and simultaneously, the colonies were characterised for their identification. The Actinomycetes and Streptomycetes colonies were characterised, and antibiotics were isolated and purified by the activated charcoal method. The reactivity of the antibiotics was tested against commercially available grades of antibiotics. As a result, we were able to isolate and characterise microbial colonies from soil. The reactivity of purified and isolated antibiotics was equivalent to commercially available antibiotics.